Understanding light microscopy / Jeremy Sanderson.

Includes bibliographical references and index.

Our eyes and the microscope -- Light -- Basic microscope optics -- Microscope design -- Ergonomics -- Optical aberrations of the microscope -- The microscope objective -- Condensers & eyepieces -- Illumination in the microscope -- Diffraction and image formation in microscopy -- Contrast generation & enhancement -- Reflected-light microscopy -- Polarised-light microscopy : part 1, theory -- Polarised-light microscopy : part 2, applied -- Fluorescence microscopy -- Fluorophores & fluorescent proteins -- Optical sectioning & confocal microscopy -- Operating the confocal microscope -- Light-sheet microscopy -- Bleed-through & spectral unmixing -- Deconvolution -- Multi-photon microscopy -- Total internal reflection fluorescence microscopy -- Frap and fret -- Colocalisation -- Super-resolution fluorescence microscopy -- Choosing a microscope platform and core imaging facilities -- Biological specimen preparation -- Materials specimen preparation -- Recording the image : part 1, theory -- Recording the image : part 2, applied.

Online resource; title from digital title page (viewed on September 27, 2019).

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum-containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more.-Gives a didactic introduction to the light microscope -Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility -Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.